Selected publications
Makukhin, N., Havelka, V., Poláchová, E., Rampírová, P., Tarallo, V., Strisovsky, K. and Misek, J. (2019)
Resolving oxidative damage to methionine by an unexpected membrane-associated stereoselective reductase discovered using chiral fluorescent probes.
FEBS Journal, in press
Tichá, A., Collis, B., and Strisovsky, K. (2018)
The Rhomboid Superfamily: Structural Mechanisms and Chemical Biology Opportunities.
Trends in Biochemical Sciences, 43: 726-739
Oikonomidi, I., Burbridge, E., Cavadas, M., Sullivan, G., Collis, B., Naegele, H., Clancy, D., Brezinova, J., Hu, T., Bileck, A., Gerner, C., Bolado, A., von Kriegsheim, A., Martin, S. J., Steinberg, F., Strisovsky, K., and Adrain, C. (2018)
iTAP, a novel iRhom interactor, controls TNF secretion by policing the stability of iRhom/TACE.
eLife 7: e35032
Goel, P., Jumpertz, T., Ticha, A., Ogorek, I., Mikles, D. C., Hubalek, M., Pietrzik, C. U., Strisovsky, K., Schmidt, B., and Weggen, S. (2018)
Discovery and validation of 2-styryl substituted benzoxazin-4-ones as a novel scaffold for rhomboid protease inhibitors.
Bioorganic and Medicinal Chemistry Letters 28, 1417-1422
Yang, J., Barniol-Xicota, M., Nguyen, M. T. N., Ticha, A., Strisovsky, K., and Verhelst, S. H. L. (2018)
Benzoxazin-4-ones as novel, easily accessible inhibitors for rhomboid proteases.
Bioorganic and Medicinal Chemistry Letters 28, 1423-1427
Goel, P., Jumpertz, T., Mikles, D. C., Ticha, A., Nguyen, M. T. N., Verhelst, S., Hubalek, M., Johnson, D. C., Bachovchin, D. A., Ogorek, I., Pietrzik, C. U., Strisovsky, K., Schmidt, B., and Weggen, S. (2017)
Discovery and Biological Evaluation of Potent and Selective N-Methylene Saccharin-Derived Inhibitors for Rhomboid Intramembrane Proteases.
Biochemistry 56, 6713-6725.
Ticha, A., Stanchev, S., Vinothkumar, K.R., Mikles, D.C., Pachl, P., Began, J., Skerle, J., Svehlova, K., Nguyen, M.T.N., Verhelst, S.H.L., Johnson, D.C., Bachovchin, D.A., Lepsik, M., Majer, P., and Strisovsky, K. (2017)
General and modular strategy for designing potent, selective and pharmacologically compliant inhibitors of rhomboid proteases.
Cell Chemical Biology 24, 1-14.
Johnson, N., Brezinova, J., Stephens, E., Burbridge, E., Freeman, M., Adrain, C., and Strisovsky, K. (2017)
Quantitative proteomics screen reveals a substrate repertoire of human rhomboid protease RHBDL2 in human cells and implicates it in epithelial homeostasis.
Scientific Reports 7, 7283.
Ticha, A., Stanchev, S., Skerle, J., Began, J., Ingr, M., Svehlova, K., Polovinkin, L., Ruzicka, M., Bednarova, L., Hadravova, R., Polachova, E., Rampirova, P., Brezinova, J., Kasicka, V., Majer, P., Strisovsky, K. (2017)
Sensitive versatile fluorogenic transmembrane peptide substrates for rhomboid intramembrane proteases.
The Journal of Biological Chemistry 292, 2703-2713.
Strisovsky, K. (2017)
Mechanism and inhibition of rhomboid proteases
Methods in Enzymology 584, 279-293.
Arutyunova, E., Panigrahi, R., Strisovsky, K., and Lemieux, M. J. (2017)
Production of recombinant rhomboid proteases
Methods in Enzymology 584, 255-278.
Arutyunova, E., Strisovsky, K., and Lemieux, M. J. (2017)
Activity assays for rhomboid proteases
Methods in Enzymology 584, 395-437.
Strisovsky, K. (2016) Rhomboid protease inhibitors: emerging tools and future therapeutics
Seminars in Cell & Developmental Biology 60, 52-62.
Wunderle, L., Knopf, J. D., Kuhnle, N., Morle, A., Hehn, B., Adrain, C., Strisovsky, K., Freeman, M. & Lemberg, M. K. (2016)
Rhomboid intramembrane protease RHBDL4 triggers ER-export and non-canonical secretion of membrane-anchored TGFalpha.
Scientific Reports 6, 27342.
Strisovsky, K. (2015)
Why cells need intramembrane proteases – a mechanistic perspective
FEBS Journal 283, 1837-45.
Wolf, E. V., Seybold, M., Hadravova, R., Strisovsky, K. & Verhelst, S. H. (2015)
Activity-Based Protein Profiling of Rhomboid Proteases in Liposomes
ChemBioChem 16, 1616-21.
Zoll, S., Stanchev, S., Began, J., Skerle, J., Lepsik, M., Peclinovska, L., Majer, P. & Strisovsky, K. (2014)
Substrate binding and specificity of rhomboid intramembrane protease revealed by substrate-peptide complex structures.
The EMBO Journal 33, 2408-2421.
Strisovsky, K. & Freeman, M. (2014)
Sharpening rhomboid specificity by dimerisation and allostery
The EMBO Journal 33, 1847-1848.
Strisovsky, K. (2013)
Structural and mechanistic principles of intramembrane proteolysis – lessons from rhomboids
FEBS Journal 280, 1579-603.
selected for issue front cover
Adrain, C., Strisovsky, K., Zettl, M., Hu, L. D. A., Lemberg, M. K. & Freeman, M. (2011)
Mammalian EGF receptor activation by the rhomboid protease RHBDL2
EMBO Reports 12, 421-427.
Pierrat*, O. A., Strisovsky*, K., Christova, Y., Large, J., Ansell, K., Bouloc, N., Smiljanic, E. & Freeman, M. (2011)
Monocyclic beta-lactams are selective, mechanism-based inhibitors of rhomboid intramembrane proteases.
ACS Chemical Biology 6, 325-335.
*equal contribution
Zettl, M., Adrain, C., Strisovsky, K., Lastun, V. & Freeman, M. (2011)
Rhomboid family pseudoproteases use the ER quality control machinery to regulate intercellular signaling.
Cell 145, 79-91.
featured as a Research Highlight in Nature Reviews Molecular Cell Biology 12, 277
Vinothkumar, K. R., Strisovsky, K., Andreeva, A., Christova, Y., Verhelst, S. & Freeman, M. (2010)
The structural basis for catalysis and substrate specificity of a rhomboid protease
The EMBO Journal 29, 3797-3809.
Strisovsky, K., Sharpe, H. J. & Freeman, M. (2009)
Sequence-specific intramembrane proteolysis: identification of a recognition motif in rhomboid substrates
Molecular Cell 36, 1048-1059.
featured as Issue Highlight
Stevenson, L. G., Strisovsky, K., Clemmer, K. M., Bhatt, S., Freeman, M. & Rather, P. N. (2007)
Rhomboid protease AarA mediates quorum-sensing in Providencia stuartii by activating TatA of the twin-arginine translocase.
PNAS 104, 1003-1008.