Selected publications

25 11 2017

Goel, P., Jumpertz, T., Mikles, D. C., Ticha, A., Nguyen, M. T. N., Verhelst, S., Hubalek, M., Johnson, D. C., Bachovchin, D. A., Ogorek, I., Pietrzik, C. U., Strisovsky, K., Schmidt, B., and Weggen, S. (2017)
Discovery and Biological Evaluation of Potent and Selective N-Methylene Saccharin-Derived Inhibitors for Rhomboid Intramembrane Proteases.
Biochemistry, in press

Ticha, A., Stanchev, S., Vinothkumar, K.R., Mikles, D.C., Pachl, P., Began, J., Skerle, J., Svehlova, K., Nguyen, M.T.N., Verhelst, S.H.L., Johnson, D.C., Bachovchin, D.A., Lepsik, M., Majer, P., and Strisovsky, K. (2017)
General and modular strategy for designing potent, selective and pharmacologically compliant inhibitors of rhomboid proteases.
Cell Chemical Biology 24, 1-14.

Johnson, N., Brezinova, J., Stephens, E., Burbridge, E., Freeman, M., Adrain, C., and Strisovsky, K. (2017)
Quantitative proteomics screen reveals a substrate repertoire of human rhomboid protease RHBDL2 in human cells and implicates it in epithelial homeostasis.
Scientific Reports 7, 7283.

Ticha, A., Stanchev, S., Skerle, J., Began, J., Ingr, M., Svehlova, K., Polovinkin, L., Ruzicka, M., Bednarova, L., Hadravova, R., Polachova, E., Rampirova, P., Brezinova, J., Kasicka, V., Majer, P., Strisovsky, K. (2017)
Sensitive versatile fluorogenic transmembrane peptide substrates for rhomboid intramembrane proteases.
The Journal of Biological Chemistry 292, 2703-2713.

Strisovsky, K. (2017)
Mechanism and inhibition of rhomboid proteases
Methods in Enzymology 584, 279-293.

Arutyunova, E., Panigrahi, R., Strisovsky, K., and Lemieux, M. J. (2017)
Production of recombinant rhomboid proteases
Methods in Enzymology 584, 255-278.

Arutyunova, E., Strisovsky, K., and Lemieux, M. J. (2017)
Activity assays for rhomboid proteases
Methods in Enzymology 584, 395-437.

Strisovsky, K. (2016) Rhomboid protease inhibitors: emerging tools and future therapeutics
Seminars in Cell & Developmental Biology 60, 52-62.

Wunderle, L., Knopf, J. D., Kuhnle, N., Morle, A., Hehn, B., Adrain, C., Strisovsky, K., Freeman, M. & Lemberg, M. K. (2016)
Rhomboid intramembrane protease RHBDL4 triggers ER-export and non-canonical secretion of membrane-anchored TGFalpha.
Scientific Reports 6, 27342.

Strisovsky, K. (2015)
Why cells need intramembrane proteases – a mechanistic perspective
FEBS Journal 283, 1837-45.

Wolf, E. V., Seybold, M., Hadravova, R., Strisovsky, K. & Verhelst, S. H. (2015)
Activity-Based Protein Profiling of Rhomboid Proteases in Liposomes
ChemBioChem 16, 1616-21.

Zoll, S., Stanchev, S., Began, J., Skerle, J., Lepsik, M., Peclinovska, L., Majer, P. & Strisovsky, K. (2014)
Substrate binding and specificity of rhomboid intramembrane protease revealed by substrate-peptide complex structures.
The EMBO Journal 33, 2408-2421.

Strisovsky, K. & Freeman, M. (2014)
Sharpening rhomboid specificity by dimerisation and allostery
The EMBO Journal 33, 1847-1848.

Strisovsky, K. (2013)
Structural and mechanistic principles of intramembrane proteolysis – lessons from rhomboids
FEBS Journal 280, 1579-603.
selected for issue front cover

Adrain, C., Strisovsky, K., Zettl, M., Hu, L. D. A., Lemberg, M. K. & Freeman, M. (2011)
Mammalian EGF receptor activation by the rhomboid protease RHBDL2
EMBO Reports 12, 421-427.

Pierrat*, O. A., Strisovsky*, K., Christova, Y., Large, J., Ansell, K., Bouloc, N., Smiljanic, E. & Freeman, M. (2011)
Monocyclic beta-lactams are selective, mechanism-based inhibitors of rhomboid intramembrane proteases.
ACS Chemical Biology 6, 325-335. *equal contribution

Zettl, M., Adrain, C., Strisovsky, K., Lastun, V. & Freeman, M. (2011)
Rhomboid family pseudoproteases use the ER quality control machinery to regulate intercellular signaling.
Cell 145, 79-91.
featured as a Research Highlight in Nature Reviews Molecular Cell Biology 12, 277

Vinothkumar, K. R., Strisovsky, K., Andreeva, A., Christova, Y., Verhelst, S. & Freeman, M. (2010)
The structural basis for catalysis and substrate specificity of a rhomboid protease
The EMBO Journal 29, 3797-3809.

Strisovsky, K., Sharpe, H. J. & Freeman, M. (2009)
Sequence-specific intramembrane proteolysis: identification of a recognition motif in rhomboid substrates
Molecular Cell 36, 1048-1059.
featured as Issue Highlight

Stevenson, L. G., Strisovsky, K., Clemmer, K. M., Bhatt, S., Freeman, M. & Rather, P. N. (2007)
Rhomboid protease AarA mediates quorum-sensing in Providencia stuartii by activating TatA of the twin-arginine translocase.
PNAS 104, 1003-1008.